PD5Web

Primer design using the PD5 library and its pd5_cli interface.

Enter the target sequence (FASTA format or plain sequence):

Options for locating the 5' end of the forward primer:
5' location range begin:
5' location range end:

Options for locating the 5' end of the reverse primer:
5' location range begin: (should be a greater value than the 5' range end, as this is the reverse primer)
5' location range end:

Length options for both primers:
Length range shortest:
Length range optimum:
Length range longest:

Options for temperature (° C):
Minimum:
Optimal:
Maximum: